culture

culture (kul´chur)

1. The propagation of microorganisms on or in media of various kinds. 2. A mass of microorganisms on or in a medium. 3. The propagation of mammalian cells, i.e., cell culture. See cell c.cultivation; [L. cultura, tillage, fr. colo, pp. cultus, to till]
batch c. a technique for large-scale production of microbes or microbial products in which, at a given point in time, the fermenter is stopped and the c. is worked up.
cell c. the maintenance or growth of dispersed cells after removal from the body, commonly on a glass surface immersed in nutrient fluid.
continuous c. a technique for production of microbes or microbial products in which nutrients are continuously supplied to the fermenter.
discontinuous c. a technique for production of microbes or microbial products in which the organisms are grown in a closed system until one nutrient factor becomes rate-limiting.
elective c. a method of isolating microorganisms capable of utilizing a specific substrate by incubating an inoculum in a medium containing the substrate; the medium usually contains substances or has characteristics that inhibit the growth of unwanted microorganisms.enrichment c;
enrichment c. elective c
hanging-block c. the propagation of microorganisms on a cube of solidified agar medium which is inoculated, attached to a cover glass, and inverted over a moist chamber or hollowed slide.
mixed lymphocyte c. See mixed lymphocyte culture test.
needle c. stab c
neotype c. neotype strain
organ c. the maintenance or growth of tissues, organ primordia, or the parts or whole of an organ in vitro in such a way as to allow differentiation or preservation of the architecture or function.
pure c. in the ordinary bacteriologic sense, a c. consisting of the descendants of a single cell.
roll-tube c. a c. in a tube of medium which has been melted and allowed to solidify while the tube is being spun; the inside of the tube is thereby coated with a thin layer of solidified medium.
sensitized c. a live c. of an organism to which a specific antiserum is added; after the mixture is incubated for several minutes (during which the antibody in the serum combines with the organisms), the excess serum is removed by means of centrifugation, washing in physiologic saline solution, and recentrifugation; the sensitized organisms may then be resuspended in physiologic saline solution.
shake c. a c. made by inoculating a liquefied gelatin or agar medium, distributing the inoculum thoroughly by agitation, and then allowing the medium to solidify in the tube in an upright position.
slant c. a c. made on the slanting surface of a medium which has been solidified in a test tube inclined from the perpendicular so as to give a greater area than that of the lumen of the tube.slope c;
slope c. slant c
smear c. a c. obtained by spreading material presumed to be infected on the surface of a solidified medium.
stab c. a c. produced by inserting an inoculating needle with inoculum down the center of a solid medium contained in a test tube.needle c;
stock c. a c. of a microorganism maintained solely for the purpose of keeping the microorganism in a viable condition by subculture, as necessary, into fresh medium.
streak c. a c. produced by lightly stroking an inoculating needle or loop with inoculum over the surface of a solid medium.
tissue c. the maintenance of live tissue after removal from the body, by placing in a vessel with a sterile nutritive medium.
type c. a type strain of microorganism preserved in a c. collection as the standard.

 

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