exonuclease (ek-so-nu´kle-as)

A nuclease that releases one nucleotide at a time, serially, beginning at one end of a polynucleotide (nucleic acid); several have been prepared from Escherichia coli, designated e. I, e. II, etc.; e. III, which removes nucleotides from 3´ ends of DNA, is used in DNA sequencing. Cf. endonuclease.


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