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(2-[(2-bis-[carboxymethyl]aono-5-methoxyphenyl)-methyl-6- methoxy-8-bis[carboxymethyl]aminoquinoline);a fluorescent compound that binds Ca++ tightly. The wavelengths of light that cause fluorescence when Ca++ is bound are longer than the wavelengths that cause fluorescence when Ca++ is not bound. When excited at two different wavelengths, the ratio of the fluorescence intensities at the two wavelengths gives the ratio of the concentrations of bound to free Ca++ Free quin-2 concentration can be measured precisely, so free Ca++ concentration can be calculated precisely. Quin-2 may be injected into cells to measure moment-to-moment changes in intracellular Ca++ concentration. See also aequorin, fura-2.
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